Question 1

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ________.

Accepted Answer

A)  isolation
B)  inoculation
C)  immunization
D)  infection
E)  contaminationF) A) and B)
G) B) and D)

Question 2

Resolution differs from contrast in microscopy in that ________.

Accepted Answer

A)  resolution refers to the ability to distinguish two cells from one another,whereas contrast refers to the ability to distinguish a cell from its surroundings
B)  contrast refers to the ability to distinguish two cells from one another,whereas resolution refers to the ability to distinguish a cell from its surroundings
C)  resolution is measured by the refractive index of light whereas contrast depends on the use of oil
D)  contrast is improved by adding oil to the specimen with the 100x objective lens and resolution is improved by adjusting the iris diaphragm control leverE) A) and D)
F) A) and C)

Question 3

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 4

In the absence of immersion oil when using the 100x objective lens,some light would be lost to scatter resulting in a blurry image.

Accepted Answer

A) True 
 B)False

Question 5

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A)  NaCl
B)  Sheep red blood cells
C)  Bile salts
D)  Thioglycollic acid
E)  PeptoneF) All of the above
G) B) and D)

Question 6

An RN is working at an urban low-income medical clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. The woman reports that she woke this morning to find she was leaking milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, explain that a vaginal speculum exam will be performed, and educate her about specimens that will be collected. Once the proper specimens are obtained and appropriately labeled, the wet mount and culturette are sent to the laboratory for processing.
-The RN understands that along with a pH test,a microscopic view is needed to perform the ferning test to detect an amniotic fluid leak.The patient sample is prepared on a glass slide and examined under 10x magnification.Which type of microscope will be used to make this observation of the patient sample?

Accepted Answer

A)  Electron microscope
B)  Light microscope
C)  Confocal microscope
D)  Fluorescent microscopeE) B) and C)
F) B) and D)

Question 7

A common medium used for growing fastidious bacteria is ________.

Accepted Answer

A)  blood agar
B)  trypticase soy agar
C)  mannitol salt agar
D)  MacConkey medium
E)  a reducing mediumF) A) and D)
G) C) and D)

Question 8

For which bacterial genus does mannitol salt agar differentiate between species?

Accepted Answer

A)  Salmonella
B)  Streptococcus
C)  Neisseria
D)  Staphylococcus
E)  EscherichiaF) B) and E)
G) A) and B)

Question 9

The real image is the reverse of the actual specimen.

Accepted Answer

A) True 
 B)False

Question 10

If a microbiologist is studying a specimen at a total magnification of 950x,what is the magnifying power of the objective lens if the ocular lens is 10x?

Accepted Answer

A)  100x
B)  950x
C)  85x
D)  850x
E)  95xF) B) and E)
G) B) and D)

Question 11

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Accepted Answer

A)  Streak plate
B)  Spread plate
C)  Pour plate
D)  All of the choices are correct.
E)  None of the choices are correct.F) A) and E)
G) A) and B)

Question 12

Comparing defined vs.complex media is analogous to comparing ________.

Accepted Answer

A)  baby formula to breast milk
B)  Coca-cola® to Pepsi®
C)  the generic version of the drug Lipitor® to the brand name produced by Pfizer Pharmaceuticals
D)  a strawberry-banana smoothie to a mixed berry smoothieE) A) and B)
F) C) and D)

Question 13

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A)  Has flexibility
B)  Holds moisture
C)  Can be inoculated and poured at a temperature that is not harmful
D)  Is solid at room temperature
E)  Is digested by most microbesF) A) and E)
G) A) and D)

Question 14

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Accepted Answer

A)  streaked
B)  poured
C)  incubated
D)  contaminated
E)  All of the choices are correct.F) A) and E)
G) A) and D)

Question 15

Differential media results in which of the following growth characteristics?

Accepted Answer

A)  Different color colonies
B)  Different media color post incubation
C)  Precipitates
D)  Gas bubbles
E)  All of the choices are correct.F) A) and D)
G) All of the above

Question 16

What type of isolation technique is most effective for the majority of applications?

Accepted Answer

A)  Pour plate
B)  Streak plate
C)  Spread plate
D)  Loop dilution
E)  Culture plateF) B) and C)
G) B) and E)

Question 17

The purpose of staining cells on a microscope slide is to ________.

Accepted Answer

A)  kill them
B)  secure them to the slide
C)  enlarge the cells
D)  add contrast in order to see them better
E)  see motilityF) A) and B)
G) C) and D)

Question 18

A confocal microscope uses visible light as its source of illumination.

Accepted Answer

A) True 
 B)False

Question 19

A 65-year-old homeless male presents to an urgent care clinic with a deep laceration on his left arm. He states that he cut his arm on an old piece of scrap metal two days prior. His wound is red, tender, hot to the touch, and has yellow drainage. The RN collects a sample of the drainage and sends it to the laboratory for microbial analysis, per provider orders.
-Microscopic analysis revealed the presence of grapelike clusters of gram-positive cocci.The RN educates the patient that the species identification of the organism will require biochemical testing,which aids in microbial identification by providing information on cellular metabolism.Which of the following statements by the patient demonstrates understanding of the nurse's teaching?

Accepted Answer

A)  Biochemical tests can determine the organism's nutrient requirements.
B)  Biochemical tests can determine the presence of enzymes in the sample.
C)  Biochemical tests can provide information about products given off during growth.
D)  Biochemical tests can provide information about the microbe's mechanism for deriving energy.
E)  All of these statements are correct.F) B) and E)
G) A) and B)

Question 20

The Gram staining procedure is best described as a(n) ________ staining technique.

Accepted Answer

A)  acid-fast or Ziehl-Neelson
B)  differential
C)  capsule
D)  flagellar
E)  simpleF) A) and B)
G) C) and D)

Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

If a microbiologist is studying a specimen at a total magnification of 950x,what is the magnifying power of the objective lens if the ocular lens is 10x?

Correct Answer
verified

Correct Answer
verified

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct Answer
verified

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ________.

Correct Answer
verified

What type of isolation technique is most effective for the majority of applications?

Correct Answer
verified

Which of the following is not a benefit of agar as a solid medium?

Correct Answer
verified

Differential media results in which of the following growth characteristics?

Correct Answer
verified

The real image is the reverse of the actual specimen.

Correct Answer
verified

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct Answer
verified

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Correct Answer
verified

A common medium used for growing fastidious bacteria is ________.

Correct Answer
verified

Mixed cultures are also referred to as contaminated cultures.

Correct Answer
verified

The purpose of staining cells on a microscope slide is to ________.

Correct Answer
verified

For which bacterial genus does mannitol salt agar differentiate between species?

Correct Answer
verified

Resolution differs from contrast in microscopy in that ________.

Correct Answer
verified

Comparing defined vs.complex media is analogous to comparing ________.

Correct Answer
verified

The Gram staining procedure is best described as a(n) ________ staining technique.

Correct Answer
verified

A confocal microscope uses visible light as its source of illumination.

Correct Answer
verified

Correct Answer
verified

In the absence of immersion oil when using the 100x objective lens,some light would be lost to scatter resulting in a blurry image.

Correct Answer
verified

Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

If a microbiologist is studying a specimen at a total magnification of 950x,what is the magnifying power of the objective lens if the ocular lens is 10x?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct AnswerverifiedShow Answer

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ________.

Correct AnswerverifiedShow Answer

What type of isolation technique is most effective for the majority of applications?

Correct AnswerverifiedShow Answer

Which of the following is not a benefit of agar as a solid medium?

Correct AnswerverifiedShow Answer

Differential media results in which of the following growth characteristics?

Correct AnswerverifiedShow Answer

The real image is the reverse of the actual specimen.

Correct AnswerverifiedShow Answer

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct AnswerverifiedShow Answer

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Correct AnswerverifiedShow Answer

A common medium used for growing fastidious bacteria is ________.

Correct AnswerverifiedShow Answer

Mixed cultures are also referred to as contaminated cultures.

Correct AnswerverifiedShow Answer

The purpose of staining cells on a microscope slide is to ________.

Correct AnswerverifiedShow Answer

For which bacterial genus does mannitol salt agar differentiate between species?

Correct AnswerverifiedShow Answer

Resolution differs from contrast in microscopy in that ________.

Correct AnswerverifiedShow Answer

Comparing defined vs.complex media is analogous to comparing ________.

Correct AnswerverifiedShow Answer

The Gram staining procedure is best described as a(n) ________ staining technique.

Correct AnswerverifiedShow Answer

A confocal microscope uses visible light as its source of illumination.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

In the absence of immersion oil when using the 100x objective lens,some light would be lost to scatter resulting in a blurry image.

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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